Sebia capillary electrophoresis: the most used capillary electrophoresis technique worldwide with fully automated protein separation at high resolution
Protein electrophoresis is a well-established technique routinely used in clinical laboratories for screening of serum and other fluids for protein abnormalities. The Sebia capillary electrophoresis (CE) instruments, CAPILLARYS and MINICAP, have been developed to provide complete automation, with fast separation and high resolution.
The Sebia systems use the principle of capillary electrophoresis in free solution.
Charged molecules are separated by their electrophoretic mobility at a specific pH in an alkaline buffer. Separation occurs according to the electrolyte pH and electroosmotic flow.
The Sebia capillary electrophoresis instruments are equipped with several parallel capillaries enabling multiple simultaneous analyses:
- CAPILLARYS 3 TERA (12 capillaries)
- CAPILLARYS 2 (8 capillaries)
- MINICAP (2 capillaries)
Each sample is diluted in a dilution buffer and the capillaries are filled with the separation buffer; samples are then injected by aspiration into the anodic end of the capillary. A high voltage protein separation is then done; direct detection and quantification of the different protein fractions is performed at a specific wavelength at the cathodic end of the capillary.
Post analysis, the capillaries are immediately cleaned with a wash solution and then refilled with buffer in preparation for the next samples.